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Volatile constituents of Teucrium flavum L. from Iran

Journal of Essential Oil Research: JEOR, Mar/Apr 2003 by Baher, Z F, Mirza, M

Abstract

The volatile constituents of Teucrium flavum L. leaves were examined by GC and GC/MS. Eighteen compounds were identified constituting about 99% of the oil. The major components were beta-caryophyllene (30.7%), gennacrene D (21.3%) and alpha-humulene (8.4%).

Key Word Index

Teucrium flavum, Labiatae, essential oil composition, beta-caryophyllene, germacrene D, alpha-humulene

Plant Name

The leaves ofT flavum were collected from shrubs grown in the National Botanical Garden in Tehran, Iran at the full flowering stage in May 2001. A voucher specimen was deposited at the Herbarium of TARI in Tehran, Iran.

Plant Parts

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Air-dried leaves of the plants (220 g) were subjected to stearn disitillation in an all-glass apparatus for 1 h to produce oil in 0.2% yield.

Previous Work

The volatile constituents of T. flavum from Croatia and Greece have been the subject of previous study (3-5).

Present Work

Gas chromatography: GC analysis was performed using a Shimadzu GC-9A gas chromatograph equipped with a DBI fused silica column (60 in x 0.25 rim, film thickness 0.25 (mu)m). Oven temperatures was 50(deg)C for 5 mini and then programmed to 270(deg)C at a rate of 4(deg)C/min; injector and detector (FID) temperatures were 280(deg)C, and carrier gas was helium with a linear velocity of 32 cm/s.

Gas chromatography/mass spectrometery: The oil was analyzed using a GC/MS (Varian 3400) equipped with a DB-- 1 fused silica column (60 in x 0.25 in in, film thickness 0.25 (mu)m) and interfaced with a Varian ion trap detector. Column temperature was programmed 50(deg)-270(deg)C at a rate of 4(deg)C/min, injector and transferline temperature was 280(deg)C and 290(deg)C, respectively; helium was the carrier gas, with a linear velocity of 31.5 cin/s split ratio 1:60, ionization energy 70 eV; scan time 1 s; mass range was 40-300 amu.

The identification of the constituents was assigned on the basis of comparison ath some available authentic compounds, GC retention time, mass spectra and retention indices. The compounds identified in the oil are listed in Table I.

Acknowledgements

The authors would like to acknowledge thefinancial support given by the Research Institute of Forests and Rangelands for this work.

References

1. V. Mozaffarian, A Dictionary of Iranian Plant Names. pp 522. Farhang Moaser, Tehran (1996).

2. P. M. Synge, Dictionary of Gardening. vol. 4, pp 2093, Oxford University Press., Oxford (1965).

3. G. Sanic, J. Petricic, N. Blazevic and M.Plazibat, Essential oil of Teucrium flavum L. from Croatia. J. Essent. Oil Res., 5,625-627 (1993).

4. B. Bellomaria, N. Arnold and G. Valentini, Essential oil of Teucrium flavum ssp. hellenicum from Greece. J. Essent. Oil Res., 10, 131-133 (1998).

5. Y. Pelissier, C. Marion, A. Malan, M.Milhau, C. Quastana and J. M. Bessiere, Volatile components of Lamiaceae from Corsica. Rivista Ital Eppos, (Numero Specials)., 520-535 (1996).

Research Institute of Forest and Rangeland, Department of Phytichemistry, PO box 13185-116, Tehran, Iran

*Address for correspondence

Received: August 2001 Revised: October 2001 Accepted: October 2001

Volatile constituents of Teucrium flavum L. from Iran

Journal of Essential Oil Research: JEOR, Mar/Apr 2003 by Baher, Z F, Mirza, M

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