Will molecular tracking reveal virus' secrets?

More than 40,000,000 people worldwide are infected with HIV, the virus that causes AIDS. While current therapies are able to repress the virus if caught early enough, scientists still are working on a cure for the deadly disease. One researcher from the University of Missouri, Columbia, has developed a unique molecular tracking system that peers into cells, uncovering how the virus assembles its "parts" to make more virus particles and spread the infection.

"Currently, scientists have been using fluorescence microscopy as an imaging system to see the different particles," explains Marc Johnson, assistant professor of microbiology and immunology. "The problem is that the resolution is nowhere near the detail we need to view individual parts of a virus. This new technique will allow us to see much more detail than before and might reveal some of the virus' hidden secrets."

HIV contains three major genes that must combine to form the virus. When HIV infects a cell, it begins the process of replicating itself by producing these three genes. Once the genes are created, they assemble to form the virus particles and then exit the cell to spread throughout the body. While current therapies prevent infection of other cells by blocking the viruses that are released, they do have some very harmful side effects. In addition, even though the viruses are unable to infect other cells, there are infected cells in the body that continue to produce more virus particles that can stay dormant in the body for years.

In addition to the scanning electron microscopy technique, Johnson is working with Deyu Fang, assistant professor of otolaryngology, who has developed a way to tag the virus parts before they assemble. Although it is a type of fluorescent, the tag does not illuminate until the virus parts are very close together. This unique aspect will allow researchers to identify specific areas of the cell where the virus assembles.

"Different locations have been implemented in the viral assembly process, but we just don't know exactly where any of this process takes place," Johnson admits. "The long-term outlook is to see if we can find a chemical that will prevent these proteins from ever assembling into the virus."

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