About 70 results for CRISPR gene editing in Academic & Research
arXiv arxiv.org Feb 4, 2016

Primer on the Gene Ontology

arXiv preprint
The Gene Ontology (GO) project is the largest resource for cataloguing gene function. The combination of solid conceptual underpinnings and a practical set of features have made the GO a widely adopted resource in the research community and an essential resource for data analysis. In this chapter, we provide a concise …
DOAJ mdpi.com Sep 1, 2022

A New Strategy for Efficient Screening and Identification of Monoclonal Antibodies against Oncogenic Avian Herpesvirus Utilizing CRISPR/Cas9-Based Gene-Editing Technology

Man Teng, Zi-Yu Zhou, Yongxiu Yao, Venugopal Nair et al. — Viruses
Marek’s disease virus (MDV) is an important oncogenic α-herpesvirus that induces Marek’s disease (MD), characterized by severe immunosuppression and rapid-onset T-cell lymphomas in its natural chicken hosts. Historically, MD is regarded as an ideal biomedical model for studying virally induced cancers. Monoclonal antib…
CORE core.ac.uk Jul 23, 2018

Research progress of gene editing technology CRISPR/Cas9 system in animal gene editing

Xu Wenlina
Gene editing technology, from the beginning of RNA interference (RNAi) technology to efficient developed enzyme technology, has been widely used in recent years. These efficient enzyme technologies include zinc finger nuclease (ZFN) technology, transcriptional activation-like effector nuclease (TALENs) technology, and …
OpenAIRE doi.org Feb 19, 2021

Comparative Study Between the CRISPR/Cpf1 and CRISPR/Cas9 systems for Multiplex Gene Editing in Maize

Chongzhi Gong, Shengchan Huang, Rentao Song, Weiwei Qi
Abstract Background: The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has been successfully used for multiplex gene editing in crops. Although CRISPR/Cas9 system has been proved to be an efficient multiplex gene editing system in crops, it was still u…
NASA ADS doi.org Nov 1, 2022

Unintended Genomic Outcomes in Current and Next Generation GM Techniques: A Systematic Review

Chu, Philomena, Agapito-Tenfen, Sarah Zanon — Plants
Classical genetic engineering and new genome editing techniques, especially the CRISPR/Cas technology, increase the possibilities for modifying the genetic material in organisms. These technologies have the potential to provide novel agricultural traits, including modified microorganisms and environmental applications.…
OpenAlex doi.org Aug 23, 2018

Cas9 immunity creates challenges for CRISPR gene editing therapies

Julie M. Crudele, Jeffrey S. Chamberlain — Nature Communications
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 is a genome-editing technology1,2 that utilizes archaeal and bacterial Cas9 nucleases to introduce double-stranded breaks in DNA at targeted sites. These breaks can be used to remove, replace, or add pieces of DNA. While not the first genome editor…
arXiv arxiv.org Feb 4, 2016

Gene Ontology: Pitfalls, Biases, Remedies

arXiv preprint
The Gene Ontology (GO) is a formidable resource but there are several considerations about it that are essential to understand the data and interpret it correctly. The GO is sufficiently simple that it can be used without deep understanding of its structure or how it is developed, which is both a strength and a weaknes…
DOAJ doi.org Mar 1, 2024

Developments and future prospects of personalized medicine in head and neck squamous cell carcinoma diagnoses and treatments

Shalindu Malshan Jayawickrama, Piyumi Madhushani Ranaweera, Ratupaskatiye Gedara Gunaratnege Roshan Pradeep, Yovanthi Anurangi Jayasinghe et al. — Cancer Reports
Abstract Background Precision healthcare has entered a new era because of the developments in personalized medicine, especially in the diagnosis and treatment of head and neck squamous cell carcinoma (HNSCC). This paper explores the dynamic landscape of personalized medicine as applied to HNSCC, encompassing both curre…
CORE core.ac.uk Jan 14, 2019

Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells.

Nakamura, Muneaki, Srinivasan, Prashanth, Chavez, Michael, Carter, Matthew A et al. — eScholarship, University of California
Repurposed CRISPR-Cas molecules provide a useful tool set for broad applications of genomic editing and regulation of gene expression in prokaryotes and eukaryotes. Recent discovery of phage-derived proteins, anti-CRISPRs, which serve to abrogate natural CRISPR anti-phage activity, potentially expands the ability to bu…
OpenAIRE doi.org Mar 1, 2015

CRISPR/Cas: A Faster and More Efficient Gene Editing System

Bin, Liu, Huifen, Xu, Jiangfang, Miao, Andy, Zhang et al.
Gene editing technology has been at its mature stage with the successful development of TALENs and CRISPR/Cas enzymes. The genetically modified endonucleases of ZFNs, TALENs, and CRISPR/Cas are widely used in the development of genetically modified cells or organisms. Among the enzymes that possess gene editing ability…
NASA ADS doi.org Jan 1, 2024

Molecular Basis and Engineering Strategies for Transcription Factor-Mediated Reproductive-Stage Heat Tolerance in Crop Plants

Sharma, Niharika, Sharma, Lakshay, Onkarappa, Dhanyakumar, Yogendra, Kalenahalli et al. — Agronomy
Heat stress (HS) is a major threat to crop productivity and is expected to be more frequent and severe due to climate change challenges. The predicted increase in global temperature requires us to understand the dimensions of HS experienced by plants, particularly during reproductive stages, as crop productivity is maj…
arXiv arxiv.org Apr 27, 2024

CRISPR-GPT for Agentic Automation of Gene-editing Experiments

arXiv preprint
The introduction of genome engineering technology has transformed biomedical research, making it possible to make precise changes to genetic information. However, creating an efficient gene-editing system requires a deep understanding of CRISPR technology, and the complex experimental systems under investigation. While…
DOAJ ieeexplore.ieee.org Apr 22, 2026

Identify Key Sequence Features to Improve CRISPR sgRNA Efficacy

Lei Chen, Shaopeng Wang, Yu-Hang Zhang, Jiarui Li et al. — IEEE Access
The CRISPR/Cas9 system is a creative and innovative gene editing biotechnology tool in genetic engineering. Although several achievements have been attained using the CRISPR/Cas9 system, it is still a challenge to avoid off-target effects and improve the editing efficacy. Previous efforts on evaluating the efficacy and…
CORE core.ac.uk Apr 19, 2017

Generation of heritable germline mutations in the jewel wasp Nasonia vitripennis using CRISPR/Cas9.

Li, Ming, Au, Lauren Yun Cook, Douglah, Deema, Chong, Abigail et al. — eScholarship, University of California
The revolutionary RNA-guided endonuclease CRISPR/Cas9 system has proven to be a powerful tool for gene editing in a plethora of organisms. Here, utilizing this system we developed an efficient protocol for the generation of heritable germline mutations in the parasitoid jewel wasp, Nasonia vitripennis, a rising insect …
OpenAIRE doi.org Jan 1, 2025

Current progress in CRISPR-Cas systems for rare diseases

Juveriya, Israr, Ajay, Kumar
The groundbreaking CRISPR-Cas gene editing method permits exact genetic code alteration. The "CRISPR" DNA protects bacteria from viruses. CRISPR-Cas utilizes a guide RNA to steer the Cas enzyme to the genome's gene editing target. After attaching to a sequence, Cas enzymes cleave DNA to insert, delete, or modify genes.…
NASA ADS doi.org Aug 1, 2024

Efficient DIPA-CRISPR-mediated knockout of an eye pigment gene in the white-backed planthopper, Sogatella furcifera

Zhang, Meng-Qi, Gong, Lang-Lang, Zhao, Ya-Qin, Ma, Yun-Feng et al. — Insect Science
Although CRISPR/Cas9 has been widely used in insect gene editing, the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small, have hard shells, and/or are difficult to collect and maintain outside of their normal environment. Such is the cas…
OpenAlex doi.org Apr 19, 2020

AAV-CRISPR Gene Editing Is Negated by Pre-existing Immunity to Cas9

Ang Li, Mark R. Tanner, Ciaran M. Lee, Ayrea Hurley et al. — Molecular Therapy
Adeno-associated viral (AAV) vectors are a leading candidate for the delivery of CRISPR-Cas9 for therapeutic genome editing in vivo. However, AAV-based delivery involves persistent expression of the Cas9 nuclease, a bacterial protein. Recent studies indicate a high prevalence of neutralizing antibodies and T cells spec…
DOAJ sciencedirect.com Sep 1, 2022

Precise detection of CRISPR-Cas9 editing in hair cells in the treatment of autosomal dominant hearing loss

Chong Cui, Daqi Wang, Bowei Huang, Fang Wang et al. — Molecular Therapy: Nucleic Acids
Gene therapy would benefit from the effective editing of targeted cells with CRISPR-Cas9 tools. However, it is difficult to precisely assess the editing performance in vivo because the tissues contain many non-targeted cells, which is one of the major barriers to clinical translation. Here, in the Atoh1-GFP;Kcnq4+/G229…
CORE core.ac.uk Jan 1, 2019

A large CRISPR-induced bystander mutation causes immune dysregulation.

Simeonov, Dimitre R, Brandt, Alexander J, Chan, Alice Y, Cortez, Jessica T et al. — eScholarship, University of California
A persistent concern with CRISPR-Cas9 gene editing has been the potential to generate mutations at off-target genomic sites. While CRISPR-engineering mice to delete a ~360 bp intronic enhancer, here we discovered a founder line that had marked immune dysregulation caused by a 24 kb tandem duplication of the sequence ad…
NASA ADS doi.org Nov 1, 2023

Microparticle-mediated CRISPR DNA delivery for genome editing in poplar

Hoengenaert, Lennart, Van Doorsselaere, Jan, Vanholme, Ruben, Boerjan, Wout — Frontiers in Plant Science
The use of CRISPR/Cas9 is currently the method of choice for precise genome engineering in plants, including in the biomass crop poplar. The most commonly used method for delivering CRISPR/Cas9 and its components in poplar is via Agrobacterium-mediated transformation, that besides the desired gene-editing event also re…
OpenAlex doi.org Jun 26, 2021

CRISPR-Cas9 In Vivo Gene Editing for Transthyretin Amyloidosis

Julian D. Gillmore, Ed Gane, Jörg Täubel, Justin Kao et al. — New England Journal of Medicine
In a small group of patients with hereditary ATTR amyloidosis with polyneuropathy, administration of NTLA-2001 was associated with only mild adverse events and led to decreases in serum TTR protein concentrations through targeted knockout of TTR. (Funded by Intellia Therapeutics and Regeneron Pharmaceuticals; ClinicalT…
arXiv arxiv.org May 11, 2023

Comparative Analysis of Machine Learning Algorithms for Predicting On-Target and Off-Target Effects of CRISPR-Cas13d for gene editing

arXiv preprint
CRISPR-Cas13 is a system that utilizes single stranded RNAs for RNA editing. Prediction of on-target and off-target effects for the CRISPR-Cas13d dependency enables us to design specific single guide RNAs (sgRNAs) that help locate the desired RNA target positions. In this study, we compared the performance of multiple …
DOAJ sciencedirect.com Aug 1, 2022

Humanized substitutions of Vmat1 in mice alter amygdala-dependent behaviors associated with the evolution of anxiety

Daiki X. Sato, Yukiko U. Inoue, Nahoko Kuga, Satoko Hattori et al. — iScience
Summary: The human vesicular monoamine transporter 1 (VMAT1) harbors unique substitutions (Asn136Thr/Ile) that affect monoamine uptake into synaptic vesicles. These substitutions are absent in all known mammals, suggesting their contributions to distinct aspects of human behavior modulated by monoaminergic transmission…
CORE core.ac.uk May 1, 2020

CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway.

Xu, Shuxiang, Kim, Jinchul, Tang, Qingshuang, Chen, Qu et al. — eScholarship, University of California
With its high efficiency for site-specific genome editing and easy manipulation, the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR associated protein 9 (CAS9) system has become the most widely used gene editing technology in biomedical research. In addition, significant progress has been ma…
OpenAIRE doi.org May 10, 2021

Comparative Study between the CRISPR/Cpf1 (Cas12a) and CRISPR/Cas9 Systems for Multiplex Gene Editing in Maize

Chongzhi Gong, Shengchan Huang, Rentao Song, Weiwei Qi
Although the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been proved to be an efficient multiplex gene editing system in maize, it was still unclear how CRISPR/Cpf1 (Cas12a) system would perform for multiplex gene editing in maize. To this end, this s…
NASA ADS doi.org Sep 1, 2020

Regulatory barriers to improving global food security

Smyth, Stuart J. — Global Food Security
Crop agriculture and food production constantly face climactic challenges to the supply of safe, nutritious food. These challenges highlight the importance of innovation resulting in improved crop technologies, capable of providing consistently increasing yields in the face of abiotic and biotic stresses. This article …
OpenAlex doi.org Apr 17, 2015

CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes

Puping Liang, Yanwen Xu, Xiya Zhang, Chenhui Ding et al. — Protein & Cell
Genome editing tools such as the clustered regularly interspaced short palindromic repeat (CRISPR)-associated system (Cas) have been widely used to modify genes in model systems including animal zygotes and human cells, and hold tremendous promise for both basic research and clinical applications. To date, a serious kn…
DOAJ life-science-alliance.org Mar 1, 2025

Genetic modulation of RNA splicing rescues BRCA2 function in mutant cells

Beatriz Anjo Lima, Ana Carolina Pais, Juliette Dupont, Patrícia Dias et al. — Life Science Alliance
This study provides proof of principle for the therapeutic potential of splicing modulation as a strategy to rescue BRCA2 function in mutant cells. Variants in the hereditary cancer-associated BRCA1 and BRCA2 genes can alter RNA splicing, producing transcripts that encode internally truncated yet potentially functional…
CORE core.ac.uk Jan 1, 2015

Developing a Gene Editing System to Study Haplodiploidy in the Jewel Wasp, Nasonia Vitripennis

Muller, Emily A. — Scholarship @ Claremont
Hymenopteran insects, which include all ants, bees and wasps, reproduce through a poorly understood form of reproduction known as haplodiploidy. A promising experimental system for understanding this developmental process is the jewel wasp, Nasonia vitripennis. A critical aspect of using Nasonia as a model is establish…
OpenAIRE doi.org Dec 1, 2023

CRISPR-based gene editing technology and its application in microbial engineering

Junwei Wei, Yingjun Li
Gene editing technology involves the modification of a specific target gene to obtain a new function or phenotype. Recent advances in clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-mediated technologies have provided an efficient tool for genetic engineering of cells and organisms. Here, we revi…
NASA ADS doi.org Oct 1, 2020

The Uproar Over Gene-Edited Babies: A Semantic Network Analysis of CRISPR on Twitter

Calabrese, Christopher, Ding, Jieyu, Millam, Benjamin, Barnett, George A. — Environmental Communication
CRISPR-Cas9, a recent gene editing technology, has potential to greatly impact medicine, agriculture, and the environment; therefore, it is increasingly important to understand the public's perceptions surrounding this technology. This study examined the main themes of CRISPR during a nine-month period on Twitter throu…
OpenAlex doi.org Dec 5, 2020

CRISPR-Cas9 Gene Editing for Sickle Cell Disease and β-Thalassemia

Haydar Frangoul, David Altshuler, Maria Domenica Cappellini, Yi-Shan Chen et al. — New England Journal of Medicine
Transfusion-dependent β-thalassemia (TDT) and sickle cell disease (SCD) are severe monogenic diseases with severe and potentially life-threatening manifestations. BCL11A is a transcription factor that represses γ-globin expression and fetal hemoglobin in erythroid cells. We performed electroporation of CD34+ hematopoie…
arXiv arxiv.org Sep 1, 2024

Gene and RNA Editing: Methods, Enabling Technologies, Applications, and Future Directions

arXiv preprint
Gene and RNA editing methods, technologies, and applications are emerging as innovative forms of therapy and medicine, offering more efficient implementation compared to traditional pharmaceutical treatments. Current trends emphasize the urgent need for advanced methods and technologies to detect public health threats,…
DOAJ mdpi.com Jun 1, 2023

Genome Editing for Cystic Fibrosis

Guoshun Wang — Cells
Cystic fibrosis (CF) is a monogenic recessive genetic disorder caused by mutations in the CF Transmembrane-conductance Regulator gene (CFTR). Remarkable progress in basic research has led to the discovery of highly effective CFTR modulators. Now ~90% of CF patients are treatable. However, these modulator therapies are …
CORE core.ac.uk Sep 1, 2019

CRISPR-Cas9-Mediated Genome Editing Increases Lifespan and Improves Motor Deficits in a Huntington's Disease Mouse Model.

Ekman, Freja K, Ojala, David S, Adil, Maroof M, Lopez, Paola A et al. — eScholarship, University of California
Huntington's disease (HD) is a currently incurable and, ultimately, fatal neurodegenerative disorder caused by a CAG trinucleotide repeat expansion within exon 1 of the huntingtin (HTT) gene, which results in the production of a mutant protein that forms inclusions and selectively destroys neurons in the striatum and o…
OpenAIRE doi.org Jun 15, 2024

CRISPR-based gene editing in plants: Focus on reagents and their delivery tools

Vida Ebrahimi, Atieh Hashemi
Introduction: CRISPR-Cas9 technology has revolutionized plant genome editing, providing precise and efficient methods for genetic modification. This study focuses on the advancements and delivery of CRISPR-Cas9 in plant gene editing. Methods: A comprehensive search in scientific databases, including PubMed, ScienceDire…
OpenAlex doi.org Nov 23, 2015

Resources for the design of CRISPR gene editing experiments

Daniel B. Graham, David E. Root — Genome biology
CRISPR-based approaches have quickly become a favored method to perturb genes to uncover their functions. Here, we review the key considerations in the design of genome editing experiments, and survey the tools and resources currently available to assist users of this technology.
arXiv arxiv.org May 8, 2023

Prokaryotic genome editing based on the subtype I-B-Svi CRISPR-Cas system

arXiv preprint
Type I CRISPR-Cas systems are the most common among six types of CRISPR-Cas systems, however, non-self-targeting genome editing based on a single Cas3 of type I CRISPR-Cas systems has not been reported. Here, we present the subtype I-B-Svi CRISPR-Cas system (with three confirmed CRISPRs and a cas gene cluster) and geno…
DOAJ doi.org Sep 1, 2023

Progress and Perspective of CRISPR‐Cas9 Technology in Translational Medicine

Ruixuan Zheng, Lexiang Zhang, Rokshana Parvin, Lihuang Su et al. — Advanced Science
Abstract Translational medicine aims to improve human health by exploring potential treatment methods developed during basic scientific research and applying them to the treatment of patients in clinical settings. The advanced perceptions of gene functions have remarkably revolutionized clinical treatment strategies fo…
CORE core.ac.uk May 14, 2019

INHIBITIONS OF HEPATITIS C VIRUS REPLICATION WITH THE HELP OF CRISPR/CAS 9 TECHNOLOGY

Dr. MuhammadUmarFarooq*, Mehwish
Hepatitis c infection caused by Hepatitis c virus (HBV) is a major world-wide health problem. Current therapeutic strategies rarely eradicate HBV infections and fail to attain complete cure. There is urgent need to develop advanced treatment strategies to successfully remove HBV infection and eliminate hidden reservoi…
OpenAIRE doi.org Apr 28, 2023

CRISPR Gene Editing of Virulent Bacteriophage ICP1

Andrew, Camilli
Tools for site-directed mutagenesis of virulent bacteriophages (phages; viruses of bacteria) have traditionally lagged those for bacteria, hindering their study. CRISPR gene editing represents a new and highly efficient method for editing virulent phage genomes. Here, I describe methods for using CRISPR gene editing fo…
NASA ADS doi.org Jun 1, 2024

Optimising commercial traits through gene editing in aquaculture: Strategies for accelerating genetic improvement

Moran, Megan N., Jones, D. B., Jensen, S. A., Marcoli, R. et al. — Reviews in Aquaculture
Aquaculture is one of the fastest-growing food production sectors. As the global human population continues to increase and further pressure is added to the prospects of achieving global food security, aquaculture is expected to play an integral role in meeting future nutrition demands. With advances in genetic technol…
arXiv arxiv.org Sep 30, 2024

FreeMask: Rethinking the Importance of Attention Masks for Zero-Shot Video Editing

arXiv preprint
Text-to-video diffusion models have made remarkable advancements. Driven by their ability to generate temporally coherent videos, research on zero-shot video editing using these fundamental models has expanded rapidly. To enhance editing quality, structural controls are frequently employed in video editing. Among these…
DOAJ journal.frontiersin.org Mar 1, 2018

Bacterial and Pneumocystis Infections in the Lungs of Gene-Knockout Rabbits with Severe Combined Immunodeficiency

Jun Song, Guoshun Wang, Mark J. Hoenerhoff, Jinxue Ruan et al. — Frontiers in Immunology
Using the CRISPR/Cas9 gene-editing technology, we recently produced a number of rabbits with mutations in immune function genes, including FOXN1, PRKDC, RAG1, RAG2, and IL2RG. Seven founder knockout rabbits (F0) and three male IL2RG null (−/y) F1 animals demonstrated severe combined immunodeficiency (SCID), characteriz…
CORE core.ac.uk Jan 1, 2017

CRISPR/Cas9–mediated deletion of CTG expansions recovers normal phenotype in myogenic cells derived from myotonic dystrophy 1 patients

Provenzano, Claudia, Cappella, Marisa, Valaperta, Rea, Cardani, Rosanna et al. — place:Cambridge
Myotonic dystrophy type 1 (DM1) is the most common adult-onset muscular dystrophy, characterized by progressive myopathy, myotonia, and multi-organ involvement. This dystrophy is an inherited autosomal dominant disease caused by a (CTG)n expansion within the 30 untranslated region of the DMPK gene. Expression of the mu…
OpenAIRE doi.org Sep 3, 2022

CRISPR-Analytics (CRISPR-A): A platform for precise analytics and simulations for gene editing

Marta Sanvicente-García, Albert García-Valiente, Socayna Jouide, Jessica Jabara-Wallace et al.
Gene editing characterization with currently available tools does not always give precise relative proportions among the different types of gene edits present in an edited bulk of cells. We have developed CRISPR-Analytics, CRISPR-A, which is a comprehensive and versatile genome editing web application tool and a nextfl…
NASA ADS doi.org Aug 1, 2023

Pho1a (plastid starch phosphorylase) is duplicated and essential for normal starch granule phenotype in tubers of Solanum tuberosum L

Sharma, Shrikant, Friberg, Martin, Vogel, Paul, Turesson, Helle et al. — Frontiers in Plant Science
Reserve starch from seeds and tubers is a crucial plant product for human survival. Much research has been devoted to quantitative and qualitative aspects of starch synthesis and its relation to abiotic factors of importance in agriculture. Certain aspects of genetic factors and enzymes influencing carbon assimilation …
arXiv arxiv.org Sep 9, 2024

DeepFM-Crispr: Prediction of CRISPR On-Target Effects via Deep Learning

arXiv preprint
Since the advent of CRISPR-Cas9, a groundbreaking gene-editing technology that enables precise genomic modifications via a short RNA guide sequence, there has been a marked increase in the accessibility and application of this technology across various fields. The success of CRISPR-Cas9 has spurred further investment a…
DOAJ frontiersin.org May 1, 2021

CRISPR-Cas9 Editing of Human Histone Deubiquitinase Gene USP16 in Human Monocytic Leukemia Cell Line THP-1

Iveta Gažová, Lucas Lefevre, Stephen J. Bush, Rocio Rojo et al. — Frontiers in Cell and Developmental Biology
USP16 is a histone deubiquitinase which facilitates G2/M transition during the cell cycle, regulates DNA damage repair and contributes to inducible gene expression. We mutated the USP16 gene in a high differentiation clone of the acute monocytic leukemia cell line THP-1 using the CRISPR-Cas9 system and generated four h…
CORE core.ac.uk Jan 1, 2020

Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae

Dermauw, Wannes, Jonckheere, Wim, Riga, Maria, Livadaras, Ioannis et al.
The use of CRISPR-Cas9 has revolutionized functional genetic work in many organisms, including more and more insect species. However, successful gene editing or genetic transformation has not yet been reported for chelicerates, the second largest group of terrestrial animals. Within this group, some mite and tick speci…
OpenAIRE pubmed.ncbi.nlm.nih.gov Aug 17, 2023

[Precision gene editing technologies based on CRISPR/Cas9: a review].

Shan, Xue, Shuya, Wang, Li, Liu, Qiaofang, Zhong et al.
Gene editing technology is a genetic operation technology that can modify the DNA sequence at the genomic level. The precision gene editing technology based on CRISPR/Cas9 system is a gene editing technology that is easy to operate and widely used. Unlike the traditional CRISPR/Cas9 system, the precision gene editing t…
NASA ADS doi.org Nov 1, 2024

Perceptions of plant breeding methods─from 'phenotypic selection' to 'genetic modification' and 'new breeding technologies'

Caradus, John R. — New Zealand Journal of Agricultural Research
Plant breeding has been closely aligned with the development of civilisations and continues to be important for the supply of nutritious food and a key factor in reducing poverty and hunger. Plant breeding uses a range of techniques for both expanding and exploiting the genetic potential of plants. However, some techni…